Enzymatic Saccharaification of Bagasse: Effects of Different Pre-Treatment Methods

Md. Zahangir Alam, Md. Moniruzzaman, SMA Sujan, Mosharof Hossain, Mohammad Shah Jamal

Abstract


About 800,000 tons of bagasse is obtained per year as a by-product from sugar industries in Bangladesh. Bagasse is one of the most readily available lignocellulosic biomass that is a potential source for ethanol production through enzymatic saccharification and hydrolysis. Pretreatment enhances saccharification by removing lignin and hemicellulose. In this study pretreatment of bagasse was done using hot compressed water (HCW), (HCW+H3PO4) and (HCW+NH3). Effects of different pretreatment parameters such as temperature, residence time, concentration of NH3 and H3PO4 were studied in detail. (HCW+H3PO4) system was found to be more effective compared to other systems investigated in this study. A maximum yield of glucose (60%) and xylose (71%) was obtained in case of (HCW+H3PO4) system at temperature180°C and 160°C and residence time of 30 min and 15 min, respectively.


Keywords


Saccharification, Bagasse, Biofuel, Pretreatment, Hot compressed water.

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References


REFERENCES

C. E. Wyman, “Biomass ethanol: Technical progress, opportunities, and commercial challengesâ€, Annu. Rev. Energy Environ. Vol. 24, pp.189-226, 1999.

S. C. Yat, A. Berger and D. R. Shonnard, “Kinetic characterization of dilute surface acid hydrolysis of timber varieties and switch grassâ€, Bioresource Technol., vol. 99, pp.3855-3863, 2008.

B. Saha, “Hemicellulose bioconversionâ€, J. Ind. Microbiol. Biotechnol., Vol. 30, pp. 279-291, 2003.

Y. Sun and J. Cheng, “Hydrolysis of lignocellulosic materials for ethanol production: a reviewâ€, Bioresource. Technol. Vol., 83, pp.1-11, 2002.

S. Pareek, J. Azuma, Y. Shimizu and S. Matsui, “Hydrolysis of newspaper polysaccharides under sulfate reducing and methane producing conditionsâ€, Biodegradation, vol. 11, pp.229-237, 2000.

C. M. Roche, C. J. Dibble and J. J. Stickel, “Laboratory-scale method for enzymatioc saccharaification of lignocelulosic biomass at high solids loadingsâ€, Biotechnol. Biofuels, vol. 2, 28, 2009.

G. Brodeur, E. Yau, K. Badal, J. Collier, K. B. Ramachandran and S. Ramakrishnan, “Chemical and Physicochemical Pretreatment of lignocellulosic Bio-mass: A Reviewâ€, Enzyme Research, doi: 10.4061/2011/787532.

S. Beszedes, M. Abel, G. Szabo, C. Hodur and Z. Laszlo, “Enhanced enzymatic saccharification of agri food solid wastes by microwave pretreatmentâ€, Annals of Faculty Engineering Hunedoara, International Journal of Engineering, pp.453-457, 2011.

M. Irfan, M. Gulsher, S. Abbas, Q. Syed, M. Nadeem and S. Baig, “Effects of Various pretreatment conditions on enzymatic saccharificationâ€, Songklanakarin J. Sci. Technol., Vol. 33, pp.397-404, 2011.

D. L. Yin, Y. Jing, W. W. AlDajani, S. D. Ulrike Tschirner, J. Schilling and R. J. Kazlauskas, “Improved pretreatment of lignocellulosic biomass using enzymatically generated peracetic acidâ€, Bioresource Technol., Vol. 102, pp.5183-5192, 2011.

A. Sluiter, B. Hames, R. Ruiz, C. Scarlata, J. Sluiter, D. Templeton and D. Crocker, “Determination of Structural Carbohydrates and Lignin in Biomass. Laboratory Analytical Procedureâ€, National Renewable Energy Laboratory Golden Co., 2006.

A. Sluiter, B. Hames, R. Ruiz, C. Scarlata, J. Sluiter and D. Templeton, “Determination of Sugars, Byproducts, and Degradation Products in Liquid Fraction Process Samples. Laboratory Analytical Procedureâ€, National Renewable Energy Laboratory Golden Co., 2005.

D. Fox, P. Gray, N. Dunn and W. Marsden, “Factors affecting the enzymic susceptibility of alkali and acid pretreated sugarcane bagasseâ€, J. Chem. Tech. Biotechnol., vol. 40, pp.117-132, 1987.

Z. Wu and Y. Y. Lee, “Ammonia recycled percolation as a complementary pretreatment　to the dilute-acid processâ€, Appl. Biochem. Biotech., vol. 63-65, pp.21-34, 1997.

Boominathan, K. and Reddy, C. A., “cAMP-mediated differential regulation of lignin peroxidase and manganese-dependent peroxidases production in the white-rot basidiomycete Phanerochaete chrysosporiumâ€, Proc. Natl. Acad. Sci., USA, 1992.

T. K. Kirk and R. L. Farrell, “Enzymatic combustion: The microbial degradation of ligninâ€, Annu. Rev. Microbiol., vol. 41, pp.465-505, 1987.




DOI (PDF): https://doi.org/10.20508/ijrer.v3i2.529.g6130

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